The expression levels of downstream genes are approximately equivalent to those of upstream genes.

<p><b>A:</b> Schematic diagram of bi-cistronic T2A-Ex. <b>B:</b> Porcine fibroblasts were transfected with vector containing a single gene, (HA)EGFP or (Myc)EGFP, or vector containing a combination of genes, (HA)EGFP-T2A-(Myc)EGFP or (Myc)EGFP-T2A-(HA)EGFP. At 24 h after transfection, the EGFP signals were analyzed by flow cytometry. Mock transfected cells were used as control (filled line). Summarized bar graphs show means ± SE of three independent replications. <b>C:</b> The cell lysates were examined by immunoblot analysis with the mouse anti-HA or mouse anti-Myc Abs, followed by the HRP-conjugated goat anti-mouse IgG. Two micrograms of each plasmid were used in this figure. These results are representative of three independent experiments.