Phylogenetic tree depicting HIV sequences from Maputo analyzed in 2009 TDR threshold survey.

<p>Tree was constructed bases on complete sequences of protease and part of RT gene (1.317 bp), using Kimura 2-parameter (K2P) model of base substitution with bootstrap analysis (100 replications) in MEGA 5.05. Nomenclature of samples herein characterized is as follows: MZ (country) and year of sequencing-patient code-region of isolation [CID: Jose Macamo Health Center and 1° de Junho Health Center]. Tree includes sequences of the HIV-1 subtypes inferred through REGA (empty circles) along with previously described subtype C in the region, India and Brazil (full circles), other subtypes (other full shapes) and outlier group CPZ, obtained from Los Alamos HIV Sequence Database Subtype Reference Alignments (http: //<a href="http://www.hiv.lanl.gov/content/index" target="_blank">www.hiv.lanl.gov/content/index</a>).