MCAF1 localizes to PML bodies through binding to SUMO2/3.

<p>(<b>A</b>) Western blot analysis of total cell lysates at the indicated times after ER: Ras induction. (<b>B</b>) Immunofluorescence analysis of MCAF1 and PML at 0 and 6 days after ER: Ras induction. Scale bar: 10 µM. (<b>C</b>) Immunofluorescence analysis of WT and D968 mutant of MCAF1 with anti-GFP antibody in IMR90 cells. Scale bar: 10 µM. (<b>D</b>) Role of MCAF1 in cellular senescence. In growing cells, MCAF1 maintains cell proliferation by activating transcription of cell cycle and histone genes (left). MCAF1 knockdown downregulates cell cycle and histone genes, and results in premature senescence (middle). In oncogene-induced senescent (OIS) cells, MCAF1 is recruited to PML bodies through binding to SUMO2/3. This may inhibit MCAF1 function to maintain expression of cell cycle genes and histones (right).