Atp6v0a2 is lower in immotile spermatozoa.

<p>(A) Western blot analysis shows the levels of Atp6v0a2 in human motile and immotile spermatozoa. (B) The bar diagram shows the quantification of Atp6v0a2 protein expression, as determined by densitometry analysis and normalized to β-actin. Immotile spermatozoa had significantly lower Atp6v0a2 expression than motile spermatozoa (P<0.01, n = 6) (*P<0.01 with respect to normal fertile donors). (C) Immunolocalization of Atp6v0a2 protein in immotile and motile spermatozoa. FITC-stained with anti-a2V antibodies (green) and DAPI nuclear stain (blue) are shown. Panel 1 indicates no expression of Atp6v0a2 protein in immotile spermatozoa. Panel 2 indicates high expression of Atp6v0a2 protein in the acrosomal region of motile spermatozoa. Data are representatives of five experiments.