Typical Chromatograms for Selectivity of HPLC-UV Method.

<p>Chromatograms of (A) blank plasma; (B) blank plasma spiked with apocynin (10 µg/mL); (C) blank plasma spiked with AN-1 (8 µg/mL); (D) blank plasma spiked with IS (80 µg/mL); (E) blank plasma spiked with apocynin (10 µg/mL), AN-1 (20 µg/mL) and IS (80 µg/mL); (F) plasma sample collected at 6 min after intravenous injection of apocynin (50 mg/kg) spiked with IS (8 µg/mL); (G) plasma sample collected at 4 min after intragastric administration of apocynin (50 mg/kg) spiked with IS (8 µg/mL): (H) plasma sample collected at 1 h after intravenous injection of AN-1 (80 mg/kg) spiked with IS (80 µg/mL); (I) plasma sample collected at 30 min after intragastric administration of AN-1 (40 mg/kg) spiked with IS (80 µg/mL). Peaks of 1, apocynin; 2, AN-1; 3, IS (carbamazepine); 4, proposed metabolite.