Downregulation of SRC-2 promotes proliferation of MCF-7 cells.

<p>MCF-7 cells with KD of SRC-2 (SRC-2 shRNA) and control shRNA MCF-7 cells (Ctr shRNA) grown in phenol red-free DMEM supplemented with charcoaled stripped FBS (5%) for three days, were seeded in E-Plate 16 and treated with either Vehicle or 8-CPT-cAMP (150 µM), IBMX (50 µM) and forskolin (10 µM) (cAMP), in the absence or presence of 17β-estradiol (E2, 10 nM) (A and B, respectively). Proliferation of the cells was measured by using the xCELLigence system monitoring cellular impedance continuously for 120 hours. The results are representative of at least three independent experiments.