Constructs of LVs carrying TK expression cassette and analysis of NT- or OT-transduced ES and iPS cells <i>in vitro</i>.

<p>(<b>A</b>) Constructs of LVs carrying thymidine kinase (TK) cDNA from herpes simplex virus driven by promoters of pluripotency genes Nanog (NT) or Oct-3/4 (OT). (<b>B</b>) ES cells were transduced with LVs (NT or OT, 300 ng of reverse transcriptase) or not transduced (WT) and further treated with 0, 10, 20, 40, 60, 80 or 100 µM GCV for 72 hours. Representative brightfield images are shown (n=3). (<b>C</b>) and (<b>D</b>) iPS cells (C) or iPS-Oct-GFP cells (D) were transduced with LVs (NT or OT, 300 ng of reverse transcriptase) or not transduced (WT) and further treated with (+) or without (-) 20 µM GCV for 72 hours. Representative brightfield images (C, D) and fluorescence images (D) are shown (n=3).