Inhibition of VPS34 attenuates autophagy and inhibits recovery of renal function in young mice during endotoxemia.

<p>Immunofluorescent microscopy (20X) of renal cortex harvested at 48 hours after LPS in young mice from 4 experimental groups: NT siRNA and saline control, VPS34 siRNA and saline control, NT siRNA and LPS, and VPS34 siRNA and LPS. (a) VPS34 (Cy3, red), actin (Alexa488, green), nucleus (Hoechst, blue). Representative images of n = 4 experiments (4 mice per experiment). (b) LC3 (Cy3, red), actin (Alexa488, green), nucleus (Hoechst, blue). Representative images of n = 4 experiments (4 mice per experiment). (c) Immunoblot analysis of renal cortex lysate detecting LC3b (16KD) in untreated mice (lanes 1 and 2) and mice treated with Non-target siRNA and LPS (lanes 3 and 4) or VPS34 siRNA and LPS (lanes 5 and 6). Representative blot is shown at 48 h timepoint after LPS from n = 2 experiments (6 mice per experiment).