FISH was performed using pAs1 (red) and pSc119.2 (green) as probes on mitotic chromosomes of ‘Mianyang11’.

<p><b>A</b> pAs1 FISH signals on chromosomes of ‘Mianyang11’, the DAPI-stained chromosomes were converted into a black and white image. <b>B</b> pSc119.2 FISH signals on chromosomes of the same cell in A, the DAPI-stained chromosomes were converted into a black and white image. <b>C</b> Merged image of A and B. Arrows indicate 3D chromosomes with pSc119.2 FISH signals. Chromosomes were counterstained with DAPI (blue).