<i>In situ</i> hybridization for HPV16-miR-H1-1 and HPV16-miR-H2-1.

<p>(A) Hematoxylin-eoxin (HE) staining, immunohistochemical staining for p16, and <i>in situ</i> (italics) hybridization for scramble (negative control), U6 (positive control), human miR-205 (positive control for cervical tissue), HPV16-miR-H1-1 (16-miR-H1-1) and HPV16-miR-H2-1 (16-miR-H2-1). Shown are two cervical intraepithelial neoplasia grade 1 (CIN 1) samples (samples 10 and 28), one squamous cell carcinoma (SCC) (sample 102), as well as normal squamous epithelium (SE) (sample 104) and normal columnar epithelium (CE) (sample 104). Arrows point to positive signals. (B) Areas of selected picture fields shown in higher magnification to depict localization and positive hybridization signal. Same numbering is used as in (A).