Compound A does not affect proteasomal degradation or translation.

<p>(A) A549 cells were preinduced with Solv or MG132 (5µM) for 1.5 h. Subsequently, cells were stimulated with Solv or CpdA (10µM) for 6h or subjected to the following temperature protocol : 2 hours at 43°C followed by 2 hours at 37°C (HS+Rec), as indicated. Total cell protein extracts were subjected to Western blot analysis detecting inducible Hsp70. Detection of PARP served as a loading control. The displayed bands were detected from one single membrane. (B) A549 cells were starved for 48h in 0% DMEM, after which these cells were treated with solvent for 48h or Compound A (CpdA) (10µM) for 2h, 6h, 24h or 48h. Total cell protein extracts were subjected to Western blot analysis detecting β-catenin. Tubulin detection served as a loading control.