Ultrastructural and histopathological analyses show similar sarcomeric structure in (t/t) and WT soleus and EDL skeletal muscles.

<p>Phenotype of mouse soleus (slow) and EDL (fast) muscle myofibrils observed by negative staining (<b>A</b>) and thin sectioning (<b>B</b> and <b>C</b>). The structure and organization of sarcomeres in both longitudinal section (B) and transverse section (C) are not disrupted in either soleus or EDL in (t/t) mice. A-band length, M-line width and Z-disc width confirm electron microscopy images, showing no significant change between (<b>t/t</b>) and WT mice (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069671#tab2" target="_blank">Table 2)</a>. I-band length variations are likely due to different lengths of the muscle during fixation. Scale bars = 200 nm. Representative hematoxylin and eosin (<b>D</b>) and Masson’s trichrome (<b>E</b>) staining images show no detectable changes in myofibrils or fibrosis in the (t/t) soleus muscles, compared to the WT control.