Additional CENP-A^Cnp1 is deposited at sub-telomeric and pericentromeric regions.

<p>ChIP-chip analysis of CENP-A^Cnp1 levels at cells with moderate expression of additional CENP-A^Cnp1 (<i>nmt81</i>-CENP-A^Cnp1) or wild-type protein levels (Endog. CENP-A^Cnp1). (<b>A</b>) Average enrichment of CENP-A^Cnp1 over different chromosomal regions (<i>cnt</i>, <i>imr</i>, <i>otr</i>) of centromeres I, II and III (X-axis, linear scale). The number of repetitive elements at different loci is indicated (x2, x4, x9). The average enrichment over 10 kb regions immediately adjacent to the centromeric <i>otr</i> repeats is also shown (10 kb region encompassing the most proximal annotated gene on each side of each centromere; <i>per1</i> and <i>rad50</i> for <i>cen1</i>, <i>rpb1</i> and <i>yox1</i> for <i>cen2</i> and <i>meu27</i> and <i>cbp3</i> for <i>cen3</i>). (<b>B</b>) Average enrichment from ChIP-chip analyses of CENP-A^Cnp1 over 120 kb regions from the left or right telomere of each chromosome. Signal intensity was averaged within 20 kb or 40 kb windows as indicated. Left panels, left telomeres; right panels, right telomeres of each chromosome.