Generation of double conditional knockout mice lacking alleles of <i>Pten</i> and <i>Survivin</i>.

<p>(A) Mouse genotype of interest was obtained by crossing mice carrying Cre transgene and floxed phosphatase region of <i>Pten</i> (exon V) with <i>floxed Survivin</i> mice. (B) Genotypes of mice (shown: 10 weeks old) were determined by PCR analysis of the tissue samples. Tail DNA extracts from cPten^-/- and <i>Pten</i>^<i>f/f</i> mice were used as positive (+) and negative (-) controls for Cre, H₂O, water. (C) Status of <i>Survivin</i> deletion was also confirmed at the protein level. This is illustrated by a representative Western blot from the ventral prostate of mice from the 10-, 21-, and 37-week groups, with β-actin serving as loading control. Prostate tissue lysates from <i>Pten </i>^<i>f/f</i><i>S</i>^f/f mice were used as normal control (ctrl).