Effect of DIDS on transport activity <i>via</i> hMCT4.

<p>(A) Oocytes were incubated for 10 min at 25°C with transport buffer of pH 5.5 containing 0.1 mM L-lactate in the absence or presence of 0.5 mM DIDS. Data are presented as means ± S.E. of three independent experiments. The background uptake values of water-injected oocytes were subtracted. (B) Oocytes were preincubated at 25°C for 10 min with/without 5 mM PLP (pH 7.5). L-lactate uptake after preincubation was measured. The oocytes were incubated for 10 min at 25°C with transport buffer of pH 7.5 containing 0.1 mM L-lactate in the absence or presence of 0.5 mM DIDS. Data are presented as means ± S.E. of three independent experiments. The background uptake values of water-injected oocytes were subtracted.