Induction of competence with CSP1 does not appreciably increase EndA-mediated DNA degradation.

<p>(A–B) Induction of both <i>comX</i> expression (A) and genetic transformation (B) in the JC0923 cells grown in THB or CTM with or without exposure to 400 ng/ml CSP1. Genetic transformation was performed with the addition of 30 ug/ml <i>rpsL</i> PCR products. Experiments were performed in triplicates and repeated three times. The means ± SD of one typical experiment are shown. (C) Integrity of the <i>rpsL</i> DNA exposed to THB-grown JC0923 cells in the presence or absence of CSP1. (D) Measurement of dsDNA integrity (from C) with ethidium bromide (excitation: 300 nm; emission: 600 nm). Experiments were performed in triplicates and repeated three times. The means ± SD of one typical experiment are shown.