Immunohistochemistry results of selected cellular factors regulated by IL-1Ra.

<p>Graph of the percent healing region (a), granulation tissue localized M1 macrophages (b), granulation tissue-localized M2 macrophages (c), M2 macrophages outside the granulation tissue (d), and granulation tissue-localized proliferating cells (d), at 5 and 11 days post-injury after PBS or IL-1Ra treatment. On day 11, IL-1Ra significantly increased the percent healing region (a). No differences within the MCL were observed at any other points. Although no differences were observed with M1 macrophages (b), IL-1Ra treatment increased M2 macrophage numbers at day 5 and/or day 11 within (c) and outside of the granulation tissue (d). Proliferating cells were significantly reduced at day 5 (e). ^a,b,c indicates within a graph, bars without a common superscript letter differ (results of Fisher’s LSD post-hoc pairwise analysis, p<.05). Values are expressed as mean cell numbers ± S.E.M.