Immunohistochemistry results of cellular and ECM factors regulated by IL-1Ra.

<p>Immunohistochemistry results showing the effects of PBS and IL-1Ra on granulation tissue localized endothelial cells (a), granulation tissue-localized blood vessel lumen (b), total myofibroblasts (c), type I procollagen (d), and type III collagen (e) at 5 and 11 days post-injury. IL-1Ra significantly increased the number of endothelial cells and blood vessel lumen on day 5 (a–b), but not day 11. In contrast, IL-1Ra significantly decreased the number of myofibroblasts at day 5 (c). No differences within the MCL were observed on day 11 (c). Regardless of day, no treatment differences were observed with type I procollagen (d) and type III collagen (e). ). ^a,b indicates within a graph, bars without a common superscript letter differ (results of Fisher’s LSD post-hoc pairwise analysis, p<.05). Values are expressed as mean density ± S.E.M. Representative micrographs of endothelial cells/blood vessel lumen (f–g) and myofibroblasts (h–i) 5 days post-injury after PBS (left column) and IL-1Ra (right column) treatment.