Effect of antiviral compounds on the replication of various CHIKV strains.

<p>Dose dependent reduction of CHIKV-induced CPE by (A) 3-deaza-adenosine, (B) 6-aza-uridine, (C) chloroquine and (D) mycophenolic acid in Vero E6 cells infected with CHIKV strains ITA07-RA1, LS3 and LS3-GFP (MOI 0.005). (E) Antiviral effect of ribavirin on CHIKV replication in BHK-21 (black lines) and Vero E6 cells (gray lines). Cell viability was normalized to untreated uninfected cells (100%). The 50% cytotoxic concentration (CC₅₀) of the compounds is indicated in the top left of each panel. (F) Dose-response curves showing the effect of five antiviral compounds on the eGFP expression in Vero E6 cells infected with CHIKV LS3-GFP (MOI 0.05) at 42 h p.i. Values were normalized to eGFP fluorescence in untreated infected cells (100%).