VZV gene transcription in infected NHNP TLAs.
- Thomas J. Goodwin (440438)
- Maureen McCarthy (440439)
- Nikolaus Osterrieder (80314)
- Randall J. Cohrs (440440)
- Benedikt B. Kaufer (341018)
Publication date
August 2013
DOIAbstract
<p>Two independent NHNP TLAs were established, infected with v63G/70R and sampled at indicated times post infection. A) qPCR analysis of VZV DNA genome copies (dORF63) and qRT-PCR of ORF63/70 transcript copies (tORF63) normalized against corresponding GAPDH copies. (B) qRT-PCR analysis of VZV ORF40 (tORF40) and ORF9 (tORF9) transcript copies normalized against GAPDH mRNA copies. Data of two independent experiments analyzed in triplicate is shown as mean relative copies and was analyzed using ΔΔCt analyses.</p
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