TGF-β1 is secreted upon stimulation with EVs in THP-1 cells.
- Jasmina S. Redzic (440520)
- Agnieszka A. Kendrick (440521)
- Karim Bahmed (440522)
- Kristin D. Dahl (440523)
- Chad G. Pearson (440524)
- William A. Robinson (217400)
- Steven E. Robinson (440525)
- Michael W. Graner (146862)
- Elan Z. Eisenmesser (245741)
Publication date
August 2013
DOIAbstract
<p>TGF-β1 secretion in THP-1 cells was measured with an ELISA assay upon stimulation with EV<sup>MCF-7</sup>, EV<sup>MDA</sup>, EV<sup>U937</sup> and EV<sup>L.6pL</sup>. Most purified EVs secrete low levels of TGF-β1 that did not contribute significantly to the overall observed secreted TGF-β1 levels. Interestingly, EV<sup>U937</sup> alone comprised high levels of TGF-β1 that were reduced upon incubation with THP-1 cells, potentially suggesting internalization of TGF-β1 that is well known <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071225#pone.0071225-Zwaagstra1" target="_blank">[36]</a>.</p
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