Identification of transgenic silkworms by molecular biology methods.

<p>(A) Amplification with ie-1-<i>neo</i>-sv40polyA-1/ie-1-<i>neo</i>-sv40polyA-2 primers (left) and <i>egfp</i>-1/<i>egfp</i>-2 primers (right) from fluorescent silkworms. (B) Identification of fluorescent silkworms with western blotting using GFP antibody (up) and by dot hybridization with a DIG-labeled ie-1 probe (down). (C) RT-PCR (up) and western blotting (down) analysis of the knockout silkworms (fluorescent silkworms) and normal silkworms.