BACH2 nuclear translocation suppresses expression of pro-survival and anti-oxidative genes in bortezomib sensitive cell lines (Jeko and SP53) but not in bortezomib resistant cell lines (Mino and Rec-1).

<p>Jeko, SP53, Mino, Rec-1 cells were treated with bortezomib (20 nM) for 24 h and total RNA was isolated by an RNA extraction kit. Purified RNA (25 ng) was used for cDNA synthesis using oligo dT primers. cDNAs of control and treated samples were used to determine the level of gene expression changes after bortezomib treatment. Expression level for each gene (<i>HO-1, MCL-1, Nrf2, CAT</i> and <i>Gss</i>) was normalized with β-actin expression. Data were expressed as mean ± SD of four experiments done in duplicates or triplicates for each gene and plotted as a relative fold of decrease or increase in bortezomib-treated samples compared to the respective gene expression in untreated controls.