Predicting functional target genes of miR-92b.

<p>(A) <i>In vivo</i> screening potential targets of miR-92b in EpCAM⁺ fetal liver cells by using customized microarray analysis. The data showed that 7.96% (34/427) of predicted target genes in miR-92b overexpressed EpCAM⁺ cells were expressed lower than that in the counterpart. And 22 genes of which that relative fold changes less than 0.5 were considered as potential function targets of miR-92b; (B) Overall, 18 of the 22 3′UTR constructs demonstrated a significant reduction in luciferase activity for 293T cells overexpressing miR-92b (293T-pri-miR-92b), especially C/EBPß; (C) Potential targets could be classified in three aspects: biological process, molecular function and cellular component by using gene function analysis software; (D) Gene ontology analysis of the predicted target genes was performed with Blast2go software. The functions of many target genes were grouped in the categories of cell differentiation, transcription factor, organ development and regulation of cell proliferation, for choosing the genes involved in differentiation that specially mediated by mir-92b.