<p>Mature monocyte-derived DCs obtained in the presence of CM from GFs displayed a significantly capacity than controls to stimulate the proliferation of allogeneic CD4+T cells. Mean ± SD of triplicates are shown and results are representative of two independent experiments. *Significant difference (P = 0.002) and **Significant difference (P<0.001).</p
<p>DC were generated under low-GM (A) and standard highGM plus IL-4 conditions (B) for 6 days, stimu...
Studies on purified blood dendritic cells (DCs) are hampered by poor viability in tissue culture. We...
<p><b>A.</b> An allogeneic mixed lymphocyte reaction (MLR) was used to determine if DCs (stimulator)...
<p>PBMCs were obtained from apheresis samples or blood, and monocytes were isolated via positive sel...
<p>(A) Monocytes from Balb/C mice were cultured for 7 days on plates with immobilized Fc or CD137-Fc...
<p>DCs were differentiated and matured (IFNγ/LPS) in RPMI medium in the presence of GM-CSF and IL-4,...
<p>mDCs <b>(1×10<sup>5</sup>)</b> were irradiated and used to induce allogeneic T cells (10<sup>6</s...
<p>Immature lowGM-DC or mature highGM/4-DC were generated to stimulate allogeneic naïve T cells. Aft...
<p>Naive CD4<sup>+</sup> T cell proliferation was assessed by thymidine incorporation (CPM) after 3 ...
Several laboratories have developed culture systems that allow the generation of large numbers of hu...
Dendritic cells (DC) are highly efficient antigen-presenting cells that initiate the primary immune ...
Dendritic cells (DC) can be generated by culture of adherent peripheral blood (PB) cells in the pres...
<p>Monocytes were cultured in RPMI medium, RLEC-CM or hNPC-CM with the indicated cytokine combinatio...
The round THP-1 cells changed to be adherent flatten cells (white arrows). Under forward scatter and...
<p>DCs were generated from peripheral blood (50 ml) collected from study patients. Mononuclear cells...
<p>DC were generated under low-GM (A) and standard highGM plus IL-4 conditions (B) for 6 days, stimu...
Studies on purified blood dendritic cells (DCs) are hampered by poor viability in tissue culture. We...
<p><b>A.</b> An allogeneic mixed lymphocyte reaction (MLR) was used to determine if DCs (stimulator)...
<p>PBMCs were obtained from apheresis samples or blood, and monocytes were isolated via positive sel...
<p>(A) Monocytes from Balb/C mice were cultured for 7 days on plates with immobilized Fc or CD137-Fc...
<p>DCs were differentiated and matured (IFNγ/LPS) in RPMI medium in the presence of GM-CSF and IL-4,...
<p>mDCs <b>(1×10<sup>5</sup>)</b> were irradiated and used to induce allogeneic T cells (10<sup>6</s...
<p>Immature lowGM-DC or mature highGM/4-DC were generated to stimulate allogeneic naïve T cells. Aft...
<p>Naive CD4<sup>+</sup> T cell proliferation was assessed by thymidine incorporation (CPM) after 3 ...
Several laboratories have developed culture systems that allow the generation of large numbers of hu...
Dendritic cells (DC) are highly efficient antigen-presenting cells that initiate the primary immune ...
Dendritic cells (DC) can be generated by culture of adherent peripheral blood (PB) cells in the pres...
<p>Monocytes were cultured in RPMI medium, RLEC-CM or hNPC-CM with the indicated cytokine combinatio...
The round THP-1 cells changed to be adherent flatten cells (white arrows). Under forward scatter and...
<p>DCs were generated from peripheral blood (50 ml) collected from study patients. Mononuclear cells...
<p>DC were generated under low-GM (A) and standard highGM plus IL-4 conditions (B) for 6 days, stimu...
Studies on purified blood dendritic cells (DCs) are hampered by poor viability in tissue culture. We...
<p><b>A.</b> An allogeneic mixed lymphocyte reaction (MLR) was used to determine if DCs (stimulator)...