Silencing of the SWI/SNF core subunit BRM phenocopies SCAI mediated effects on invasive cell migration.

<p>(A) MDA-MB-435 cells were transfected with indicated siRNAs. After 48 h cells were analyzed for their invasive properties using a 3D matrix (matrigel). Representative images show confocal sections of invaded cells stained for F-actin (red) and DAPI (blue) at 20 µm distance to the transwell membrane. Three-dimensional reconstruction shows a side view of experiments with the location of invaded cells with respect to the transwell membrane (dashed line). A quantification of three independent experiments (+/−s.d.) is shown in (B) for MDA-MB-435 cells and in (C) for MDA-MB-231 cells. (D) MDA-MB-435 cells were processed for immunoblot analysis after 48 h of siRNA treatment and the abundance of BRM protein was assessed using the indicated antibodies.