Increased triglyceride accumulation in adipocyte is associated with increased intracellular 4-HNE contents.

<p>A, Adipogenesis in 3T3-L1 fibroblasts was induced by standard adipogenesis-inducing mix. Intracellular 4-HNE-protein adducts formation were detected by Western blot on day 3, 5, 7, and 13 during the process of adipogenesis. Significant elevations in intracellular 4-HNE contents were observed on day 7 (D7) in comparison to these at day 3 and 5, and the elevations continued to day 13 (D13). B, Adipogenesis in 3T3-L1 fibroblasts was induced by standard adipogenesis-inducing mix. Time-course changes of intracellular triglyceride (TG) levels were determined on day 0, 3, 5, 7, and 13 during the process of adipogenesis. All values are denoted as Means ± SD from three or more independent batches of cells. *p<0.05 vs. D0. C & D, Oleate, at the concentration of 0.2 mM was next added into the culture medium on day 6 after the induction of adipogenesis and TG contents were determined 24 hours later (day 7) by Oil Red O Staining and biochemical assay in adipocytes as described in the materials and method. UT, untreated 3T3-L1 cells (day6); OA, Oleate. All values are denoted as Means ± SD from three or more independent batches of cells. *p<0.05 vs. D6. D, Oleate, at the concentration of 0.2 mM was next added into the culture medium on day 6 after the induction of adipogenesis and intracellular 4-HNE adducts formation was determined by Western blot on day 7. UT, untreated 3T3-L1 cells (day6); OA, Oleate.