Change in the gene expression of EGFP⁺ cells after MCAO.

<p>(<b>A</b>) Changes in the gene expression of highly expressed (log₂ relative average expression above 2.3 from all data) astrocytic/NG2 glia markers and membrane proteins at P50 (blue), D3 (orange), D7 (green), and D14 (red). The listed genes show at least 2-fold up (+) or 2-fold downregulation (−) between sequential stages. (<b>B</b>) PCA clustering of cells from all post-ischemic stages and P50 (data mean-centered along genes). The stages are indicated in color (P50 blue, D3 orange, D7 green, and D14 red), and the three groups identified by SOM are indicated by symbols (squares for B3; circles for B1 and triangles for B2). (<b>C</b>) The incidence of cells from the three subpopulations at post-ischemic stages and P50. The genes under the table indicate highly expressed genes (log2 relative average expression above 1.8 and at least 40% positive cells) in particular subpopulations. (<b>D</b>) The distribution of control and post-ischemic stages in individual subpopulations.