ERManI is a target of miR-125b.

<p><b>A</b>. Illustration of the predicted target sequence of miR-125a and miR-125b located at the 3’-UTR of ERManI mRNA. “CUCAGGGA” on ERManI transcript represents the seed sequence. <b>B</b>. Western blotting of ERManI and actin (used as loading control) in PLC/PRF5 cells transfected with indicated amounts of control miRNA, miR-125a, or miR-125b. <b>C</b>. The percentage of ERManI expressed in MCF7 cells transfected with 12.5pmol miR-125a or miR-125b as compared to that transfected with equal amounts of control miRNA. The data were based on the densitometry measurement of the protein bands detected by western blotting. Error bars represent one standard deviation from three repeated samples. <b>D</b>. The luciferase constructs carrying the wild-type 3’UTR of ERManI or the ERManI 3’UTR with a mutated seed region were transfected into HeLa cells, and the luciferase activities were measured 24hr after transfection. The average activities from three repeated samples were used to calculate the percentage of inhibition. Error bars represent the standard error of the mean from three independent experiments.