Long-term multipassage culture of hBM-MSCs in different surface/media conditions.

<p>Cells were cultured for 8 sequential passages using the indicated conditions. Seeding density at each passage was 7000 cells/cm². Cumulative cell number (A), doubling time (B), cumulative population doublings (PDs) (C), and % viability (D) across the multiple passages are shown. The error bars in D represent the average +/− STDEV from 7 serial passages. Statistical analyses by an ANOVA followed by the Tukey’s method performed on the doubling time (7 passages) and % viability (8 passages) found that doubling time of TCT/with serum was significantly higher and that the % viability for TCT/with serum was significantly lower compared to the ECM and Synthemax Surface serum-free conditions (p-value = 0.000).