Profiles of density of HCV produced in different hepatoma cell lines.

<p>HuH-7 (<b>A</b>), Hep3B (<b>B</b>), HepG2-CD81 (<b>C</b>) and PLC/PRF/5 (<b>D</b>) were electroporated with <i>in vitro</i> transcribed RNA of the JFH1-CS-A4-RLuc genome containing mutations R1373Q/C2441S. The supernatants of each electroporated cell lines were recovered six days post-electroporation and overlaid on 10 to 50% (weight/volume) iodixanol gradients. After a 24 h ultracentrifugation, sixteen fractions were collected and analyzed for HCV RNA quantity and infectivity on naive HuH-7 cells (assessed by measuring <i>Renilla</i> Luciferase activities). The results are expressed as percentages of total infectivity or HCV RNA and are reported as means of two independent experiments.