Reduced mGluR5 expression in the layer V of mPFC of Cx-mGluR5 KO mice.

<p>Low-magnification images of double immunofluorescence with anti-mGluR5 and anti-CB1 antibodies on mPFC sections of control and Cx-mGluR5 KO mice (<b>A1</b>, <b>B1</b>). <b>A2</b> and <b>B2</b> depict merged staining of mGluR5, CB1 and DAPI within the white square from the left panel. The expression of mGluR5 was dramatically reduced in the cortex of Cx-mGlu5 KO mice (<b>B3</b>), while the CB1 expression was similar to littermate controls (A4, B4). (Roman numerals indicated different layers in mPFC. Scale bars in A1, B1∶500 µm; A2, A3, A4, B2, B3, B4∶50 µm.