Formate production activities of NADPH-FDHs.

<p>The reaction solution contained 1.67 mg/mL of FDH, 1 mM NAD(P) H, 0.05% Triton X-100 and 1.1–1.3 mg/ml dissolved CO₂. The concentration of formate was determined by a HPLC equipped with a Shim-pack SCR-102H column and a conductivity detector, CDD-10A_VP (Shimadzu, Kyoto, Japan). Open circles, closed circles, open triangles and closed triangles represent data of combinations of WTs with NADPH, mutants with NADPH, WTs with NADH, and mutants with NADH, respectively. All measurements were performed in triplicate.