The alteration of mitochondria phenotypes associated with the <i>AluYb8MUTYH</i>.

<p>(A, B) The leukocytic mtDNA content was associated with the <i>AluYb8MUTYH</i> variant. The relative amounts of mt-CO 1 and mt-tRNA^Leu content in the newborn, young, middle-aged and aged groups are shown. The mean mtDNA/nDNA ratio in the <i>A/A</i> genotype group of healthy newborn infants was set to 1. Error bars indicate the standard error of the mean. The number of participants is shown in brackets. <i>P</i>-values are indicated (multinomial logistic regression). (C) The respiring mitochondria mass in cultured cells (passage number 5) of C:2, C:3 and C:5 was detected by flow cytometry. The results were confirmed in triplicate, and similar results were obtained when other cultured fibroblast-like cells with different <i>AluYb8MUTYH</i> genotypes were used. Error bars represent the mean ± SD with n = 3. The geometric mean value of fluorescence intensity in the <i>A/A</i> cells was set to 1. <i>P</i>-values are indicated (2-sided t-test). (D) The respiring mitochondria mass were confirmed in cultured cells by confocal microscopy. Representative images from independent experiments are shown. DAPI (4′, 6-diamidino-2-phenylin-dole, Sigma) nuclear staining is shown. Scale bar, 20 µm. Five images of each fibroblast-like cell culture, with the indicated genotype, from the same experiment were quantified. Fluorescence was quantified with Image J software (NIH). A plot of the relative values of the respiring mitochondria levels is displayed in the lower right corner. The geometric mean of the fluorescence intensity in the <i>A/A</i> cells was set to 1. The values are presented as the mean ± SD (n = 5). <i>P</i>-values are indicated (2-sided t-test). The result was confirmed in triplicate. (E) Relative oxygen consumption capacity of intact cells (<i>P/P</i> and <i>A/A</i> cells). The oxygen consumption rate in <i>A/A</i> cells was set to 1. The data are the mean ± SD from at least two independent experiments. <i>P</i>-value is indicated (2-sided t-test).