Chorioallantoic membrane (CAM) assay.

<p>At day 9 of embryonic development, matrigel droplets with or without 50,000 hDPSC were applied onto the CAM of a chicken embryo (black arrow, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071104#pone-0071104-g005" target="_blank">Figure 5a</a>). 72 hours later, pictures were taken (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071104#pone-0071104-g005" target="_blank">figure 5c–e</a>). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071104#pone-0071104-g005" target="_blank">Figure 5c</a> is a control matrigel droplet, while <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071104#pone-0071104-g005" target="_blank">Figure 5d</a> is a droplet containing 50,000 hDPSC. White scale bars represent 1 mm. To assess angiogenesis, two circles (with a radius of 1.5 and 2 mm, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071104#pone-0071104-g005" target="_blank">figure 5e</a>), were digitally positioned around the matrigel droplets and the blood vessels intersecting these circles were counted double blind. Graph 5b: hDPSC were able to significantly increase the number of capillaries intersecting both circles. This assay was repeated 4 times with hDPSC of 4 different donors to gain a database of at least 27 individual eggs. Values are represented as means ± S.E.M.