<p>Means of cycle threshold (CT) values for amplification of <i>agtrpa1</i> and <i>rps7</i> were shown (n=2). Quantitative RT-PCR was performed on cDNA isolated from whole larvae receiving AgTRPA1, non-specific siRNA and buffer injection. Relative mRNA abundance + S.E.M was plotted with data normalized to non-specific siRNA treatment using PFAFFL method.</p
The quantitative real-time reverse transcription polymerase chain reaction (Q-RT-PCR) technique has ...
<p>Total RNA was isolated from wing imaginal discs of third instar larvae at 2 or 18 hr after exposu...
<p>(A) Expression profile of <i>vvl</i> during the late larval and prepupal stages of <i>Tribolium.<...
<p>cDNA libraries from larval antennae, heads and bodies were generated by extracting mRNA followed ...
<p>The mRNA levels of target genes were monitored by qRT-PCR at 24, 48, 72, 96 and 120 h after injec...
<p>Third instar larvae were intrahemocoelically injected with target and control gene siRNA duplexes...
<p>Early second-instar larvae of <i>G</i>. <i>viridula</i> were injected with double-stranded RNA (d...
<p>Total RNA was extracted from transgenic pupae that expressed only the ECFP marker, and from wild-...
<p>(<b>A</b>) qRT-PCR analysis of the OBP mRNA expression levels in Tc larvae of different weight, u...
<p><i>(A)</i> RT-PCR with primers RATZEB1 and RATZEB2 on total RNA from adults (ad), larvae (lar) an...
<p>Results of quantitative real-time PCR analysis are shown. Transcription level of PRAT after injec...
<p>The expression of these proteins was silenced by feeding dsRNA to <i>A. aegypti</i> larvae. The e...
<p>RNA expression of indicated target genes in GIST T1 cells after knockdown with corresponding siRN...
<p>Expression of, <i>Diptericin (dipt), drosocin (drc), cecropinA (cecA) and attacinA (attA),</i> in...
siRNA larvicide #427 was identified in soaking screens for mosquito larval lethal genes. Although co...
The quantitative real-time reverse transcription polymerase chain reaction (Q-RT-PCR) technique has ...
<p>Total RNA was isolated from wing imaginal discs of third instar larvae at 2 or 18 hr after exposu...
<p>(A) Expression profile of <i>vvl</i> during the late larval and prepupal stages of <i>Tribolium.<...
<p>cDNA libraries from larval antennae, heads and bodies were generated by extracting mRNA followed ...
<p>The mRNA levels of target genes were monitored by qRT-PCR at 24, 48, 72, 96 and 120 h after injec...
<p>Third instar larvae were intrahemocoelically injected with target and control gene siRNA duplexes...
<p>Early second-instar larvae of <i>G</i>. <i>viridula</i> were injected with double-stranded RNA (d...
<p>Total RNA was extracted from transgenic pupae that expressed only the ECFP marker, and from wild-...
<p>(<b>A</b>) qRT-PCR analysis of the OBP mRNA expression levels in Tc larvae of different weight, u...
<p><i>(A)</i> RT-PCR with primers RATZEB1 and RATZEB2 on total RNA from adults (ad), larvae (lar) an...
<p>Results of quantitative real-time PCR analysis are shown. Transcription level of PRAT after injec...
<p>The expression of these proteins was silenced by feeding dsRNA to <i>A. aegypti</i> larvae. The e...
<p>RNA expression of indicated target genes in GIST T1 cells after knockdown with corresponding siRN...
<p>Expression of, <i>Diptericin (dipt), drosocin (drc), cecropinA (cecA) and attacinA (attA),</i> in...
siRNA larvicide #427 was identified in soaking screens for mosquito larval lethal genes. Although co...
The quantitative real-time reverse transcription polymerase chain reaction (Q-RT-PCR) technique has ...
<p>Total RNA was isolated from wing imaginal discs of third instar larvae at 2 or 18 hr after exposu...
<p>(A) Expression profile of <i>vvl</i> during the late larval and prepupal stages of <i>Tribolium.<...