RT-PCR analysis of flippases and scramblases expression in mouse eggs.

<p>Total mouse egg RNA was subjected to RT-PCR using specific primers for each tested enzyme. Products were separated on 2% agarose gels and stained with ethidium bromide (lane <b>C</b>). As positive controls, total RNA from different tissues were used for each tested enzyme (lane <b>A</b>): epididymis for <i>Atp8a1</i>, testis for <i>ATP8a2</i>, liver for <i>Plscr1</i>, kidney for <i>Plscr2</i>, lung for <i>Plscr3</i> and <i>Plscr4</i>, and cumulus cells for <i>Cd52</i>. As negative controls, water was used as template for the reverse transcription (lane <b>B</b>) and during the amplification reaction (lane <b>D</b>). The expression of <i>Cd52</i> was evaluated to control contamination with cumulus cells. Results are representative of 3 independent egg RNA isolations.