PCBP1 directly binds to p63 3′UTR

<p>. (A) PCBP1 interacts with <i>p63</i> transcripts <i>in vivo</i>. Cell lysates from HaCaT cells were collected and immunoprecipitated with PCBP1 antibody or control IgG, followed by RT-PCR analysis to determine the level of <i>ΔNp63</i> transcripts in control IgG and anti-PCBP1 immunocomplexes. The levels of <i>p21</i> and <i>Actin</i> transcripts were measured as positive and negative controls, respectively. (B) Schematic presentation of <i>p63α</i> transcript and the location of probes. CUE regions are shown in shaded box. (C) PCBP1 directly binds to p63 3′UTR. REMSA was performed by mixing a ³²P-labeled RNA probes (A, B, C, or p21 probe) with recombinant GST or GST-fused PCBP1. The arrow indicates RNA-protein complexes. (D) REMSA assay was performed by mixing a ³²P-labeled RNA probe A along with or without excess amount (50-fold) of unlabeled p21 or probe A.