<p>(A) Expression of GR first variants expression in liver, 18S are used as the reference gene; (B) Expression of total GR mRNA and protein in liver; (C) Percentage of exon 1–9/10 mRNA relative to total GR mRNA in liver of piglets. Values are mean ± SEM, n = 5. *, <i>P</i><0.05, **, <i>P</i><0.01, compared with LW piglets.</p
<p><b>a)</b> The RT-PCR products (1080 bp) of <i>SLA-MIC2</i> exons 2 to 6 in different tissues, amp...
<p>The vertical axis represents the Log2 fragments per kilobase of exon length million mapped reads ...
BackgroundGene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in bio...
<p>Blank bars represent Large White (LW) piglets; filled bars represent Erhualian (EHL) piglets. Res...
<p>(A) Hepatic GR gene expression in both male and female newborn piglets. (B) The content of hepati...
<p>(A) Relative real-time RT-PCR analysis of GFP mRNA expression from newborn to maturity in founder...
<p>(A) Expression of p53 mRNA in liver tissue, 18S was used as the reference gene; (B) Nuclear p53 p...
<p>mRNA abundance of <i>FAS</i>, <i>SCD-</i>1, <i>ELOVL</i>-6 and <i>CoA</i> in the liver of newborn...
<p>Numbering is with respect to the translation start site within exon 2. The start of the porcine e...
<p>(A) Locations of the 3′ boundary of the porcine alternative first exons of porcine GR. (B) Map of...
<p>(A) Sequence of porcine GR promoter 1–9/10 analyzed. Shaded sequences represent identified porcin...
<p>The vertical axis of the figure was presented as the target genes copies (<i>GPR41</i> and <i>GPR...
<p>Histone H1 was used as a loading control. The nuclear contents of the transcription factors are p...
<div><p>Glucocorticoids are vital for life and regulate an array of physiological functions by bindi...
<p>The 2<sup>-ΔΔCt</sup> value of DS piglets is set at for each gene 1 to allow comparisons.</p
<p><b>a)</b> The RT-PCR products (1080 bp) of <i>SLA-MIC2</i> exons 2 to 6 in different tissues, amp...
<p>The vertical axis represents the Log2 fragments per kilobase of exon length million mapped reads ...
BackgroundGene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in bio...
<p>Blank bars represent Large White (LW) piglets; filled bars represent Erhualian (EHL) piglets. Res...
<p>(A) Hepatic GR gene expression in both male and female newborn piglets. (B) The content of hepati...
<p>(A) Relative real-time RT-PCR analysis of GFP mRNA expression from newborn to maturity in founder...
<p>(A) Expression of p53 mRNA in liver tissue, 18S was used as the reference gene; (B) Nuclear p53 p...
<p>mRNA abundance of <i>FAS</i>, <i>SCD-</i>1, <i>ELOVL</i>-6 and <i>CoA</i> in the liver of newborn...
<p>Numbering is with respect to the translation start site within exon 2. The start of the porcine e...
<p>(A) Locations of the 3′ boundary of the porcine alternative first exons of porcine GR. (B) Map of...
<p>(A) Sequence of porcine GR promoter 1–9/10 analyzed. Shaded sequences represent identified porcin...
<p>The vertical axis of the figure was presented as the target genes copies (<i>GPR41</i> and <i>GPR...
<p>Histone H1 was used as a loading control. The nuclear contents of the transcription factors are p...
<div><p>Glucocorticoids are vital for life and regulate an array of physiological functions by bindi...
<p>The 2<sup>-ΔΔCt</sup> value of DS piglets is set at for each gene 1 to allow comparisons.</p
<p><b>a)</b> The RT-PCR products (1080 bp) of <i>SLA-MIC2</i> exons 2 to 6 in different tissues, amp...
<p>The vertical axis represents the Log2 fragments per kilobase of exon length million mapped reads ...
BackgroundGene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in bio...