Reduced chemotaxis towards serum or PDGF-AA of WIP

<p>^{−<b>/</b>−}<b>murine fibroblasts.</b> Control (WIP^+/+) and WIP^−/− murine fibroblasts were loaded onto the upper chamber of the Transwell and the percentage of cells chemotacting to the lower well was calculated. Cells were assayed for chemotaxis towards 15% serum (<b>a</b>), 5 µM LPA (<b>b</b>), 50 g/ml EGF (<b>c</b>), 20 ng/ml FGF (<b>d</b>) and 50 ng/ml PDGF-AA (<b>e</b> and <b>f</b>). Reconstitution assays were performed by lentiviral-mediated expression of WIP-GFP or control GFP in WIP^−/− cells and overexpression assays by transduction of control (WIP^+/+) fibroblasts with GFP or WIP-GFP. * p<0,05; ** p<0,001.