WIP deficiency does not affect fibroblast morphology or protein content.

  • Inmaculada Banon-Rodriguez (443111)
  • Julia Saez de Guinoa (443112)
  • Alejandra Bernardini (443113)
  • Chiara Ragazzini (443114)
  • Estefania Fernandez (443115)
  • Yolanda R. Carrasco (443116)
  • Gareth E. Jones (285340)
  • Francisco Wandosell (1238)
  • Ines Maria Anton (443117)
Publication date
August 2013

Abstract

<p><b>a</b> Representative western blot of WIP, N-WASP and Nck expression in soluble lysates of lung-derived fibroblasts from WIP<sup>+/+</sup> and WIP<sup>−/−</sup> mice. Numbers indicate relative expression levels of each protein to GAPDH content and control fibroblasts determined by densitometry. GAPDH labeling confirmed equivalent protein loading control. <b>b</b> FACS analysis of forward and side scatter in control (WIP<sup>+/+</sup>) and WIP<sup>−/−</sup> fibroblasts. <b>c</b> Phase-contrast images from plated control (WIP<sup>+/+</sup>) and WIP<sup>−/−</sup> murine fibroblasts do not show evident morphological differences between both populations. Scale bar 50 µm.</p

Extracted data

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