EBF1 differentially regulates ERα and ERβ.

<p>(A,B) MCF-7/C4–12 cells were transfected with ERα in the presence or absence of EBF1 and then treated with 10 nM E2 or Vehicle control. (A) Association between ERα and EBF1 was confirmed by co-immunoprecipitation assay. (B) ERα protein levels were measured on Western blots (bands from each row were from the same gel but were rearranged to fit the order similar to that shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071355#pone-0071355-g007" target="_blank">figure 7A</a> on ERβ). (C) Transcriptional activities of different ER constructs were measured with the ERE-Luciferase reporter assay in the presence and absence of EBF1; percentages in parentheses represent how much EBF1 affected each construct, as measured by subtracting luciferase signal in EBF1 transfected samples from their vector controls. (D) Effect of EBF1 on ER transcriptional activities measured by subtracting the luciferase signal in EBF1 transfected samples from their vector controls; bar graph represents the average of three independent experiments; error bars represent standard deviations.