ERβ negatively regulates cell proliferation in an E2 dependent-manner.

<p>C4–12/Flag.ERβ cells were treated with 10 nM E2 for 3 days. Cell confluency, detected by the Incucyte FLR live content imaging system (Essen Bioscience), was used to measure cell proliferation. Bar graph represents fold change relative to day 0. (Error bar = standard deviation).