DSB-2 and SUN-1 S8P persistence requires axis proteins HTP-1 and HTP-3.

<p>(A and B) Immunofluorescence images of gonads of indicated genotypes from the distal pre-meiotic region to end of pachytene, stained with DAPI and antibodies that recognize DSB-2 and SUN-1 S8P. The zone of DSB-2 and SUN-1 S8P-positive nuclei is not extended in the <i>htp-1</i> and <i>htp-3</i> mutants, which lack major components of the meiotic chromosome axes, despite the fact that these mutants are impaired in formation of interhomolog COs.