Quantitation of the DSB-2 positive zone in WT and meiotic mutants.

<p>Bar graph showing the extent of the region of DSB-2 positive nuclei in germ lines of indicated genotypes. The presence/absence of DSB-2 signals was assessed in the portion of the germ line extending from the onset of DSB-2 staining to the end of the pachytene region. The extent of the DSB-2 positive zone was defined as the percentage of continuous rows of nuclei in which all or most nuclei exhibited DSB-2 staining out of total rows of nuclei in the scored region. Representative germ lines were imaged and scored: 5 for WT and 3 for each of the meiotic mutants. Error bars show standard deviation.