In vitro wound closure in primary cultures of mouse corneal epithelial cells (MCE).

<p>Confluent monolayers of MCE cells of the indicated genotype were wounded by a pipette tip and monitored by time-lapse microscopy for 24 hrs. Wound healing was determined as the average linear speed of the wound edge over 8 hrs. <i>Sulf1^−/−</i> MCE migrated at a reduced rate compared to wild-type (WT) and <i>Sulf2^−/−</i> cells. Data are expressed as means+SEM, n = 3 per group. Statistical significance was evaluated with a Student t-test. *p = 0.023, **p = 0.009.