Histological and bacteriological examination of the brains of recipients after adoptive transfer.

<p>The mice were sacrificed (n = 3, independent duplicate experiments) on day 2 post-transfer. A total of 8 wax blocks were prepared as shown. Histological changes in the meninges were observed only in blocks 2 and 3 (A). Representative images of intensive and moderate meningeal neutrophil infiltration and vascular congestion with rare cell infiltration are shown (10× or 20×, left column; 400×, right column, magnified from the black square). The position of the superior sagittal sinus (S) and the presence of neutrophils (N) or abscesses (arrow) are indicated (B). The distribution (%) of each histological category (intensive, moderate and rare neutrophil infiltration) is shown (C). Simultaneously, CSF samples were collected and seeded into culture bottles. The proportion (100%) of positive <i>B. pseudomallei</i> cultures obtained from the tested mice is shown. The lower limit of CSF detection was 200 CFU/ml (D).