Oroxylin A promotes hepatic cell proliferation in CCl₄-induced acute liver injury mice.

<p>Immunohistochemical staining for proliferating cell nuclear antigen (PCNA) was carried out as previously described. PCNA staining liver sections in the mice without (A) or with oroxylin A administration (B) at day 2 after CCl₄ treatment. The control group showed few PCNA+ hepatocytes in centrilobular areas, but oroxylin A administration demonstrated numerous PCNA+ hepatocytes surrounding the edge of hepatocellular necrosis. PCNA staining liver sections from the control (C) and oroxylin A administration (D) at day 3 after CCl₄ treatment. The control shows much more numbers of PCNA+ cells compared to oroxylin administration in the view. (E) PCNA+ cells in the liver sections were measured. At least six 12 mm² tissue sections were counted for each mouse. Values represent mean ± SE (n = 6). (*), (**), and (***) for <i>P</i><0.05, <i>P</i><0.01 and <i>P</i><0.001 respectively.