Internalisation of D25scFv, D34scFv, D25p and D34p in αvβ6-expressing cells.

<p>Internalisation of bound D25scFv (A,B) and D34scFv (D, E) was assessed at 0 mins (A,D) and 45 mins (B,C,E,F) in αvβ6-expressing cells (A,B,D,E) and detected using anti-myc antibody. In control cells (C,F) only anti-myc antibody was used. The scFvs both were internalised by αvβ6-expressing cells and some located to the nucleus. The absence of nuclear staining with anti-myc antibody alone suggests this was a true nuclear localisation. Internalisation of D25p (G, H, I) and D34p (J, K, L) was assessed in αvβ6-expressing cells ((G,H,J,K) and αvβ6-negative cells (I, L) at the times indicated. Both peptides were internalised only by αvβ6-expressing cells. The scale bar shown represents 20 µm.