Immunofluorescent TUNEL and PCNA assays at 4 weeks after irradiation.

<p>(A and B) TUNEL assays revealed few TUNEL-positive apoptotic cells among normal SGs, but significant numbers in irradiated SGs. hAdMSC administration significantly reduced TUNEL-positive apoptotic cell numbers in the hAdMSC group as compared with the irradiated group. (A and C) PCNA assays conducted at 4 weeks after transplantation showed that proliferation indices tended to be higher for hAdMSC-treated SGs than for untreated SGs. The ratios of TUNEL- and PCNA-positive cells versus all DAPI-positive cells were calculated in random tissue sections. At least three random tissue sections per gland from ten mice were chosen for evaluation. Bar = 50 µm, ^*<i>P</i><0.05, ^**<i>P</i><0.01.